Cytogenetics / FISH

Karyotype

Karyotyping, or chromosomal analysis, can provide insights into diagnosis, prognosis, and prediction of disease in many acute and chronic leukemias and lymphomas. Traditional G-banding for metaphase analysis with specific cell culturing and read out by two independent cytotechnologists ensures optimal results.

  • Best-in-industry turnaround time (3 days)
  • Cell-specific culture (myeloid, B-cell, T-cell, and plasma cell)
  • <1% culture failure*

FISH

With its ability to bind to only specific parts of a chromosome and spotlight abnormalities through their tagged fluorescence on cells when viewed under a fluorescence microscope, fluorescence in situ hybridization (FISH, also known as molecular cytogenetics), can identify abnormalities that may be missed at a macro level in karyotype, but can captured on a micro level in FISH.

  • Best-in-industry turnaround time (2 days)
  • Disease-focused panels for myeloid, lymphoid, and plasmacytic disorders including cell enrichment
  • Complete suite of hematopoietic FISH probes for custom panels and follow up
  • Global and TC services
  • HER2, ALK, ROS1, and UroVysion for solid tumor analysis
Cytogenetics / Karyotyping Notes
Chromosome Analysis, Bone Marrow, UNSTIMULATED Myeloid 24 and 48 hour cultures
Chromosome Analysis, Bone Marrow, STIMULATED
(T- and B-Cell, Lymphoproliferative Disorders, Plasma Cell)
T-Cell 72 hour culture, if necessary
B-Cell 120 hour culture, if necessary
Chromosome Analysis, Blood UNSTIMLUATED Myeloid 24 and 48 hour cultures
Chromosome Analysis, Blood STIMULATED
(T- and B-Cell, Lymphoproliferative Disorders, Plasma Cell)
T-Cell 72 hour culture, if necessary
B-Cell 120 hour culture, if necessary

*With appropriate cell-specific culture, karyotype, and FISH triage.

Hematologic FISH Panels and Probes
ALL FISH Panel
  • BCR/ABL t(9;22)
  • MLL (11q23)
  • ETV6/RUNX1 t(12;21)
  • IGH (14q32)
Mantle Cell Lymphoma FISH CCND1/IGH t(11;14)
AML FISH Panel
  • RUNX1/RUNX1T1 t(8;21)
  • MLL (11q23)
  • PML/RARA t(15;17)
  • CBFB (16q22)
  • RARA (17q21)
Marginal Zone / MALT Lymphoma FISH Panel
  • CEP3
  • CCND1/IGH t(11;14)
  • D721/D7S486 (7p11.1-q11.1/7q31)
  • BIRC3/MALT1 (11p22.1/18q21.3)
Burkitt’s Lymphoma FISH Panel
  • IGH/MYC t(8;14)
  • IGH-BA (14q32.2)
MDS FISH Panel
  • D5S23:D5S721/CSF1R (5p/q)
  • D721/D7S486 (7p11.1-q11.1/7q31)
  • +8 (8cen)
  • ETV6 (12p13)
  • D20S108 (20q)
CLL FISH Panel
  • ATM (11q22)
  • CEP12
  • D13S319 (13q14.3)
  • TP53 (17p13)
MPN FISH Panel
  • +8 (8cen)
  • BCR/ABL t(9;22)
  • ETV6 (12p13)
  • D13S319 (13q14.3)
  • D20S108 (20q)
CML FISH BCR/ABL t(9;22) Myeloma FISH Panel
  • 1pTEL/1p36/1q25
  • CEP9
  • IGH/CCND1 t(11;14)
  • D13S319/LAMP1 (13q-)
  • CEP15
  • TP53 (17p13)
Eosinophilia FISH Panel
  • CHIC2
  • PDGFRβ (5q32)
  • FGFR1 (8p11.2)
  • PCM1/JAK2 t(8;9)
Myeloma FISH Reflex panel
  • IGH-BA (14q32.2)
  • IGH/FGFR3 t(4;14)
  • IGH/MAF t(14;16)
  • IGH/MAFG t(14;20)
Follicular Lymphoma FISH Panel
  • BCL6 (3q27)
  • MYC-BA (8q24)
  • IGH/BCL2 t(14;18)

All FISH probes are available individually or in custom panels.

Solid Tumor FISH Panels and Probes
NSCLC FISH Panel
  • ALK (2p23)
  • ROS1 (6q22.1)
  • With reflex to ROS1
UroVysion Bladder FISH Panel
  • CEP3
  • CEP7
  • 9p21
  • CEP17
HER2 FISH
  • HER2
  • CEP17

All FISH probes are available individually or in custom panels.

"While we have many laboratories to choose from, we consider siParadigm to be the most reliable. Contact with a hematopatholgist is rapid and informative."

Barry Kaplan, MD, PhD
Oncologist/Hematologist, Queens Medical Assoc.

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We consistently strive to set the model for exactly how a reference laboratory should engage with both physicians and patients.

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All billing is treated as in-network regardless of insurance company.

Patients are only responsible for payments according to their in-network benefit program, co-insurance and/or deductible for laboratory services.

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siParadigm is accredited by CLIA (Clinical Laboratory Improvement Amendments) and certified by CAP (College of American Pathologists).

We also hold select state licensure where required.