Specimen Requirements
| Test Category | Specimen Type | Specimen Requirements | Specimen Container | Transport Conditions | Specimen Stability | Notes |
|---|---|---|---|---|---|---|
| Morphology | Bone Marrow Core | 1-2cm in length | 10% Neutral Buffered Formalin (NBF) | Room Temperature | Indefinitely |
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| Bone Marrow Clot | 10% Neutral Buffered Formalin (NBF) | Room Temperature | Indefinitely |
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| Bone Marrow Aspirate Slides | Five (5) unstained, unfixed aspirate smears | Plastic Slide Holder | Room Temperature | Indefinitely |
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| Test Category | Specimen Type | Specimen Requirements | Specimen Container | Transport Conditions | Specimen Stability | Notes |
|---|---|---|---|---|---|---|
| Histology (General H&E Stains) | Fresh Tissue | Primarily tumor tissue | 10% Neutral Buffered Formalin (NBF) | Room Temperature | Indefinitely |
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| FFPE Tissue | FFPE Cassette | Room Temperature | Indefinitely | Include cold pack in extreme heat. | ||
| Unstained Slides | Five (5) unstained, formalin-fixed slides | Plastic slide holder | Room Temperature | Indefinitely | Include cold pack in extreme heat. | |
| Histology (IHC and Special Stains) | Fresh Tissue | Primarily tumor tissue | 10% Neutral Buffered Formalin (NBF) | Room Temperature | Indefinitely |
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| FFPE Tissue | FFPE Cassette | Room Temperature | Indefinitely | Include cold pack in extreme heat. | ||
| Unstained Slides | Two (2) unstained, formalin-fixed slides | Plastic slide holder | Room Temperature | Indefinitely | Include cold pack in extreme heat. | |
| Histology - Breast Panel | FFPE Tissue | Block | FFPE Cassette | Room Temperature | Indefinitely |
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| Unstained Slides | Ten (10) unstained, formalin-fixed slides | Plastic slide holder | Room Temperature | Indefinitely | Include cold pack in extreme heat. | |
| Histology -Lymphoma, Myeloma and Neuroendocrine Panels | FFPE Tissue | Block | FFPE Cassette | Room Temperature | Indefinitely | Include cold pack in extreme heat. |
| Unstained Slides | Ten (10) unstained, formalin-fixed slides | Plastic slide holder | Room Temperature | Indefinitely | Include cold pack in extreme heat. |
| Test Category | Specimen Type | Specimen Requirements | Specimen Container | Transport Conditions | Specimen Stability | Notes |
|---|---|---|---|---|---|---|
| Leukemia/Lymphoma Immunophenotyping | Bone marrow aspirate | 1-2 mL | Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. | Ship at room temperature (18-25°C) if testing will occur within 8 hours of collection, otherwise ship with cold packs to maintain temperature at 2-8°C | 48 Hours | Hemolyzed, frozen or clotted samples may be rejected. |
| Peripheral blood | 2-5 mL | Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. | Ship at room temperature (18-25°C) if testing will occur within 8 hours of collection, otherwise ship with cold packs to maintain temperature at 2-8°C | 48 Hours | Hemolyzed, frozen or clotted samples may be rejected. | |
| Fresh bone marrow core biopsy | 1 - 2 cm core immersed in provided 5mL sterile transport media (RPMI 1640 & 1% penicillin-streptomycin) | Sterile transport media (RPMI 1640 & 1% penicillin-streptomycin) | Ship at room temperature (18-25°C) if testing will occur within 8 hours of collection, otherwise ship with cold packs to maintain temperature at 2-8°C | 24 Hours | Immerse tissue completely in transport media. Label with patient name, DOB, and collection date. DO NOT use RPMI past expiration date. | |
| Fresh Unfixed Tissue | 1 cm3 (marble-sized) piece of tissue immersed in provided 5mL sterile transport media (RPMI 1640 & 1% penicillin-streptomycin) | Sterile transport media (RPMI 1640 & 1% penicillin-streptomycin) | Ship at room temperature (18-25°C) if testing will occur within 8 hours of collection, otherwise ship with cold packs to maintain temperature at 2-8°C | 24 Hours | Immerse tissue completely in transport media. Label with patient name, DOB, and collection date. DO NOT use RPMI past expiration date. | |
| Fluids | 10mL of fluid in sterile transport media (RPMI 1640 & 1% penicillin-streptomycin) | Sterile transport media (RPMI 1640 & 1% penicillin-streptomycin) | Ship at room temperature (18-25°C) if testing will occur within 8 hours of collection, otherwise ship with cold packs to maintain temperature at 2-8°C | 24 Hours | Label with patient name, DOB, and collection date. DO NOT use RPMI past expiration date. | |
| Leukemia/Lymphoma Immunophenotyping - Myeloma MRD | Bone marrow aspirate | 2-5 mL | Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. | Ship at room temperature (18-25°C) if testing will occur within 8 hours of collection, otherwise ship with cold packs to maintain temperature at 2-8°C | 48 Hours | Hemolyzed, frozen or clotted samples may be rejected. Include pathology report for initial diagnosis of myeloma disease. Must assay ≥500,000 viable leukocytes for myeloma MRD testing |
| Peripheral blood | 2-5 mL | Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. | Ship at room temperature (18-25°C) if testing will occur within 8 hours of collection, otherwise ship with cold packs to maintain temperature at 2-8°C | 48 Hours | Hemolyzed, frozen or clotted samples may be rejected. Include pathology report for initial diagnosis of myeloma disease. Must assay ≥500,000 viable leukocytes for myeloma MRD testing | |
| Paroxysmal Nocturnal Hemoglobinuria (PNH) | Bone marrow aspirate | 1-2 mL | Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. | Ship at room temperature (18-25°C) if testing will occur within 24 hours of collection, otherwise ship with cold packs to maintain temperature at 2-8°C | 48 Hours | Hemolyzed, frozen or clotted samples may be rejected. |
| Peripheral blood | 2-5 mL | Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube. | Ship at room temperature (18-25°C) if testing will occur within 24 hours of collection, otherwise ship with cold packs to maintain temperature at 2-8°C | 48 Hours | Hemolyzed, frozen or clotted samples may be rejected. |
FISH
| Test Category | Specimen Type | Specimen Requirements | Specimen Container | Transport Conditions | Specimen Stability | Notes |
|---|---|---|---|---|---|---|
| FISH | Bone Marrow & Peripheral Blood | 1~2ml | Green top (Sodium Heparin) tube OR Lavender top (EDTA) tube, Smears or Cytospin | Room Temperature | 120 Hours | DO NOT FREEZE |
| Formalin Fixed Paraffin Embedded Tissue (FFPET) | Block | FFPE Cassette | Room Temperature | Indefinitely | Include cold pack in extreme heat. | |
| Unstained, formalin-fixed slides. One (1) slide per FISH probe and one (1) additional for Pathological evaluation. | Plastic slide holder | Room Temperature | Indefinitely | Include cold pack in extreme heat. |
HER2 (ERBB2) by FISH
| Test Category | Specimen Type | Specimen Requirements | Specimen Container | Transport Conditions | Specimen Stability | Notes |
|---|---|---|---|---|---|---|
| FISH | Formalin Fixed Paraffin Embedded Tissue (FFPET) | Block | FFPE Cassette | Room Temperature Include cold pack in extreme heat. | Indefinitely | Specimens subject to HER2 (ERBB2) testing should be fixed in 10% neutral buffered formalin for at least six hours and up to 72 hours. The volume of formalin should be at least 10 times the volume of the specimen. Decalcification solutions with strong acids should not be used. Specimens should be immersed in fixative within one hour of the biopsy or resection. Include cold ischemic time and fixation time on requisition. |
| Unstained, formalin-fixed slides. Two (2) slides for FISH testing and one (1) additional for pathological evaluation. | Plastic slide holder | Room Temperature Include cold pack in extreme heat. | Indefinitely | Include cold pack in extreme heat. |
| Test Category | Specimen Type | Specimen Requirements | Specimen Container | Transport Conditions | Specimen Stability | Notes |
|---|---|---|---|---|---|---|
| Karyotype (cytogenetics) | Bone Marrow (peripheral blood will be accepted in certain disease as CLL or if circulating blood containing more than 10% blasts) | Bone Marrow is ≥1.5 mL and the Peripheral blood is ≥2 mL if 10% or more blasts are present | Green top (Sodium Heparin) | Room Temperature | 72 Hours | No Frozen, Hemolyzed, Washed, in Syringes, in broken container or in different anti-coagulant other than sodium heparin. |
| Test Category | Specimen Type | Specimen Requirements | Specimen Container | Transport Conditions | Specimen Stability | Notes |
|---|---|---|---|---|---|---|
| Hematology - Automated Complete Blood Count (CBC) Test with Differential | Peripheral blood | 2-5 mL | Lavender top (EDTA) tube. | Ship with cold packs to maintain temperature at 2-8°C | 48 Hours | Hemolyzed, frozen or clotted samples may be rejected. |


